Evaluation of BacT/Alert 3D liquid culture system for recovery of mycobacteria from clinical specimens using sodium dodecyl (lauryl) sulfate-NaOH decontamination

Authors
Carricajo, A Fonsale, N Vautrin, AC Aubert, G
Citation
A. Carricajo et al., Evaluation of BacT/Alert 3D liquid culture system for recovery of mycobacteria from clinical specimens using sodium dodecyl (lauryl) sulfate-NaOH decontamination, J CLIN MICR, 39(10), 2001, pp. 3799-3800
Citations number
9
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
10
Year of publication
2001
Pages
3799 - 3800
Database
ISI
SICI code
0095-1137(200110)39:10<3799:EOB3LC>2.0.ZU;2-1
Abstract
A total of 52 mycobacterial isolates were recovered from 1,197 clinical spe cimens decontaminated by a sodium dodecyl (lauryl) sulfate (SDS)-NaOH proto col. Of these, 94% were recovered with the BacT/Alert 3D system (Organon Te knika, Durham, N.C.) and 79% were recovered on Lowenstein-Jensen (LJ) mediu m. Mean times to detection of organisms of the Mycobacterium tuberculosis c omplex (n = 47) were 22.8 days with LJ medium and 16.2 days with the system . The BacT/Alert 3D system is a rapid and efficient detection system which can be used with an SDS-NaOH decontamination procedure.