Bd. Lynn et al., Identification of sequence, protein isoforms, and distribution of the hyaluronan-binding protein RHAMM in adult and developing rat brain, J COMP NEUR, 439(3), 2001, pp. 315-330
The protein RHAMM (for "receptor for hyaluronan-mediated motility"; CD168)
is a member of the hyaladherin family of hyaluronan-binding proteins. RHAMM
has a role in cell signaling, migration, and adhesion via interactions wit
h hyaluronan, microtubules, actin, calmodulin, and components of the extrac
ellular regulated kinase (erk) signaling pathway. Based on previous finding
s of potentially similar roles in neural cells in culture, we investigated
the molecular characteristics, protein expression profile, and distribution
of RHAMM in rat brain. Reverse transcriptase-polymerase chain reaction (RT
-PCR) using RNA isolated from adult rat brain yielded a single RHAMM sequen
ce of 2.1 kilobases encoding a protein of 82.4 kDa. RHAMM is subject to alt
ernate splicing in other systems, but no RT-PCR evidence was found for spli
ce variants in brain, although our analysis does not rule out this possibil
ity. The amino acid sequence displayed homology with human and murine RHAMM
(74% and 80%, respectively) but contained only one copy of a 21-amino-acid
sequence that is repeated five times in the murine homologue. By using ant
i-RHAMM antibodies, several RHAMM isoforms were identified in brain. Immuno
histochemically, RHAMM was found in the vast majority of neurons and in man
y oligodendrocytes throughout brain, with heterogeneous levels among cell p
opulations, and was confined to the somata and initial processes of these c
ells. RHAMM was detected in neurons of cerebral cortex and most subcortical
and brainstem structures at postnatal day 1 and exhibited an adult distrib
ution pattern by postnatal day 5. High levels were detected in oligodendroc
ytes by postnatal day 10. The widespread expression of RHAMM in adult and d
eveloping brain implies a role for this protein and its ligand hyaluronan i
n key events of cell signaling and cytoskeletal regulation in the CNS. (C)
2001 Wiley-Liss, Inc.