The separation of stereoisomers from oligomers of low molecular weight polystyrene on a carbon clad zirconia column using a reversed phase multidimensional HPLC system
Ap. Sweeney et al., The separation of stereoisomers from oligomers of low molecular weight polystyrene on a carbon clad zirconia column using a reversed phase multidimensional HPLC system, J LIQ CHR R, 24(17), 2001, pp. 2559-2581
The two dimensional separation of low molecular weight polystyrene oligomer
s and stereoisomers was achieved using a liquid chromatographic system desi
gned for 'heart-cutting' multidimensional chromatography. The first separat
ion process incorporated a C18 column and a methanol mobile phase to separa
te the oligomers, whilst the second separation process utilised the change
in selectivity associated with a carbon clad zirconia stationary phase and
an acetonitrile mobile phase for the separation of the isomers. The two dim
ensional separation that was achieved using this automated system was compa
rable to the individual separations following manual sample manipulation in
each individual separation step. Furthermore, the multidimensional LC syst
em was shown to be highly reproducible in routine continuous operation over
an eight hour period.