Determination of oxolinic acid in marine sediment by hplc with fluorescence detection

A high performance liquid chromatographic (HPLC) method based on fluorescen ce detection was developed for determination of oxolinic. acid in marine se diment. Sediment was extracted with a mixture of sodium hydroxide and dimet hyl sulphoxide and cleaned up by solid phase extraction (SPE). The limit of detection was 1.6 mug/kg. The recoveries were 85 +/-6% (mean +/- SD) at a level of 10 mug/kg and 82 +/-4% (mean +/- SD) when samples containing < 1.6 -190 <mu>g/kg oxolinic acid were spiked with 50 mug/kg oxolinic acid. The r elative repeatability standard deviation was 5.5% at levels of 2-10 mug/kg and 2.5% at a level of 25 mug/kg.