Comparative study of the chiral resolution of beta-blockers on cellulose tris (3,5-dimethylphenylcarbamate) phases in normal and reversed phase modes

The chiral separation of beta -blockers on cellulose tris (3,5-dimethylphen ylearbamate) columns is described. The separation power of a Chiracel OD no rmal phase column, a Chiracel OD-RH reversed phase column, and a Chiracel O DRH reversed phase narrow-bore column were compared. Hexane-2-propanol-diet hylamine mixtures were used as mobile phase in the normal phase mode and so dium perchlorate buffer containing different amounts of acetonitrile in the reversed phase mode. Sixteen mu -blockers were resolved under normal phase conditions and eleven under reversed phase conditions. The values of a and Rs on normal phase column ranged from 4.46 to 1.10 and 10.04 to 0.97, resp ectively. The values of a and Rs on reversed phase columns varied from 4.04 to 1.04 and 3.98 to 0.40 (on normal bore column) and 4.36 to 1.06 and 2.26 to 0.40 (on narrow bore column).