Identification and simultaneous analysis of harmane, harmine, harmol, isovitexin, and vitexin in Passiflora incarnata extracts with a novel HPLC method

Authors
Grice, ID Ferreira, LA Griffiths, LR
Citation
Id. Grice et al., Identification and simultaneous analysis of harmane, harmine, harmol, isovitexin, and vitexin in Passiflora incarnata extracts with a novel HPLC method, J LIQ CHR R, 24(16), 2001, pp. 2513-2523
Citations number
27
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
10826076 → ACNP
Volume
24
Issue
16
Year of publication
2001
Pages
2513 - 2523
Database
ISI
SICI code
1082-6076(2001)24:16<2513:IASAOH>2.0.ZU;2-U
Abstract
A high performance liquid chromatographic method for the simultaneous analy sis of two flavonoids (iso-vitexin and vitexin), and three indole alkaloids (harmane, harmine, and harmol) was developed. This method was then utilise d to quantitate levels of these five constituents in methanolic extracts of Australian Passiflora incarnata. HPLC analysis was performed using a Waters(TM) Novapak C-18 (150 x. 4 mm 4 mum) column, with a gradient solvent system of methanol-water-acetic acid. Detection was achieved by PDA UV (254 nm) and fluorescence (excitation 254 nm, emission 414 nm), utilising the external standard method to obtain quan tification.