Modification of specific lysine residue of cytochrome c(3) with 2,4,6-trini
torobenzenesulfonic acid sodium salt dehydrate (TNBS) was carried out. By c
omparing the hydrogen evolution rate with modified cytochrome c(3) and nati
ve cytochrome c(3) catalyzed by hydrogenase, the role of lysine residue of
cytochrome c(3) was discussed. The modified lysine residue was responsible
for the intermolecular electron transfer from cytochrome c(3) to hydrogenas
e. (C) 2001 Elsevier Science B.V. All rights reserved.