Preparation of monofunctional and phosphorescent palladium(II) and platinum(II) coproporphyrin labeling reagents

A method is described for preparation of monosubstituted derivatives of pla tinum and palladium coproporphyrin-I. The method is based on chemical diffe rentiation of one of the four alkoxycarbonyl groups of the starting copropo rphyrin-I tetraalkyl ester by selective acid hydrolysis. The hydrolysis res ults after chromatographic purification to pure mono hydroxycarbonyl coprop orphyrin, which can be subsequently coupled with a chemical species carryin g a functional group of interest. The described method is used for preparat ion of phosphorescent metalloporphyrin labeling reagents having an isothioc yanato reactive group. Copyright (C) 2001 John Wiley & Sons, Ltd.