Q. Zhong et al., Efficient c-kit receptor-targeted gene transfer to primary human CD34-Selected hematopoietic stem cells, J VIROLOGY, 75(21), 2001, pp. 10393-10400
We have previously reported effective gene transfer with a targeted molecul
ar conjugate adenovirus vector through the c-kit receptor in hematopoietic
progenitor cell lines. However, a c-kit-targeted recombinant retroviral vec
tor failed to transduce cells, indicating the existence of significant diff
erences for c-kit target gene transfer between these two viruses. Here we d
emonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovir
us vector enables retroviral transduction. This finding suggests the requir
ement of endosomalysis for successful c-kit-targeted gene transfer. Further
more, we show efficient gene transfer to, and high transgene expression (66
%) in, CD34-selected, c-kit(+) human peripheral blood stem cells using a c-
kit-targeted adenovirus vector. These findings may have important implicati
ons for future vector development in c-kit-targeted stem cell gene transfer
.