Pt. Lacava et al., RAPD profile and antibiotic susceptibility of Xylella fastidiosa, causal agent of citrus variegated chlorosis, LETT APPL M, 33(4), 2001, pp. 302-306
Aims: The aim of this study was to evaluate the diversity of Xylella fastid
iosa isolated from citrus trees affected by Citrus Variegated Chlorosis (CV
C).
Methods and Results: The antibiotic susceptibility by agar disc diffusion a
nd minimum inhibitory concentration (MIC) methods was observed for all drug
evaluated, except for penicillin-G. Genetic diversity by RAPD analysis rev
ealed three major groups (citrus, coffee and grapevine), being the citrus g
roup more similar with the coffee group than with the grapevine group.
Conclusions, Significance and Impact of the Study: This study highlights th
e possibility to use these antibiotics susceptibility as markers in the dev
elopment of a cloning vector and penicillin-G could be used as a selective
marker for the isolation of X. fastidiosa from citrus plants.