SEPARATION OF CHIRAL AMINO-ACIDS BY MICELLULAR ELECTROKINETIC CHROMATOGRAPHY WITH DERIVATIZED CYCLODEXTRINS

The chiral separation of several amino acid (AA) enantiomers derivatiz ed with naphthalene-2,3-dialdehyde (NDA) was achieved by use of cyclod extrin-modified micellar electrokinetic chromatography (CD-MEKC). Both neutral hydroxypropyl beta-cyclodextrin (HP-beta-CD) and charged carb oxymethyl beta-cyclodextrin (CM-beta-CD) were used as buffer additives for optical resolution of derivatized amino acids, The order of eluti on and the mechanism of separation for different CDs were explained by considering important chemical equilibria in the sodium dodecyl supha te, CD and amino acid system. Furthermore, the importance of SDS for t he separation of a mixture of AAs, and the effect of CD and analyte co ncentration on the resolution will be discussed. (C) 1997 by John Wile y & Sons, Ltd.