Proper positioning of mitotic spindles ensures equal allocation of chromoso
mes to daughter cells. This often involves interactions between spindle and
astral microtubules and cortical actin(1). In yeast and Caenorhabditis ele
gans, some of the protein machinery that connects spindles and cortex has b
een identified but, in most animal cells, this process remains mysterious.
Here, we report that the tumour suppresser homologue APC2 and its binding p
artner Armadillo both play roles in spindle anchoring during the syncytial
mitoses of early Drosophila embryos. Armadillo, alpha -catenin and APC2 all
localize to sites of cortical spindle attachment. APC2-Armadillo complexes
often localize with interphase microtubules. Zeste-white 3 kinase, which c
an phosphorylate Armadillo and APC, is also crucial for spindle positioning
and regulates the localization of APC2-Armadillo complexes. Together, thes
e data suggest that APC2, Armadillo and alpha -catenin provide an important
link between spindles and cortical actin, and that this link is regulated
by Zeste-white 3 kinase.