In vitro and in vivo multiplication of virus-free 'Spunta' potato

In vitro shoots of Solanum tuberosum cv 'Spunta' were subcultured on liquid MS media containing 0.0, 0.5, 1.0, 1.5 and 2.0 mg/l of benzyl adenine (BA) or kinetin. Significant reduction in stem and internode length was observe d by increasing BA and kinetin concentrations. BA LIP to 1.0 and 1.5 mg/l s howed an increase in number of proliferated shoots and number of nodes per flask. Single node cuttings were rooted on solid media containing NAA, IBA, or LAA @ 0.0, 0.5, 1.0, 1.5, 2.0 ino, individually with or Without 1.0 mg/ l GA(3). Sucrose concentration from 30 to 90 g/l gave significant enhanceme nt of microtuberization. In vitro rooted shoots were successfully acclimati zed. Cuttings, 3.0 cm from glasshouse grown plants were successfully rooted by treating 0.5 cm base with 1 g/l IBA- 0.5 g/l IAA for five seconds.