Nested PCR was used as a diagnostic tool to investigate the presence of Ech
inococcus multilocularis tapeworm in protected species of carnivores in Slo
vakia. Twenty-three faecal samples from wolves, four samples from bears and
three samples from lynx from the Muranska Planina mountains and eight samp
les from wolves from the Bukovske Vrchy mountains were examined. E. nultilo
cularis DNA was detected in two samples of wolves' faeces from the Muranska
Planina mountains and one sample from the Bukovske Vrchy mountains. Examin
ation of bears' and lynx faecal samples gave negative results. The detectio
n of E. multilocularis in wolves has extended the range of its known defini
tive hosts in Europe.