High throughput isolation of DNA and RNA in 96-well format using a paint shaker

Authors
Michaels, SD Amasino, RM
Citation
Sd. Michaels et Rm. Amasino, High throughput isolation of DNA and RNA in 96-well format using a paint shaker, PL MOL B RE, 19(3), 2001, pp. 227-233
Citations number
1
Categorie Soggetti
Plant Sciences
Journal title
PLANT MOLECULAR BIOLOGY REPORTER
ISSN journal
07359640 → ACNP
Volume
19
Issue
3
Year of publication
2001
Pages
227 - 233
Database
ISI
SICI code
0735-9640(200109)19:3<227:HTIODA>2.0.ZU;2-F
Abstract
Most steps in plant nucleic acid isolation are easily adapted to 96-well fo rmat; however, tissue disruption typically is performed on samples individu ally and often is the rate-limiting step in sample processing. We have foun d that DNA and RNA isolation from Arabidopsis tissue can be carried out in 96-well format using a paint shaker fitted with an adapter for tissue disru ption.