Total amount of ECP per eosinophil as indicator for the activity state of eosinophils

Eosinophil cationic protein (ECP) serum concentrations yield additional inf ormation regarding the activity of allergic diseases because ECP serum conc entrations correlate very well with blood eosinophil counts. We established spontaneous serum ECP release per eosinophil during in vitro coagulation a mounting to 0.03-0.5 pg per eosinophil, When comparing the ECP per eosinoph il ratio with the eosinophil concentration, a decreasing trend of released ECP in serum was observed towards higher eosinophil counts. The total amoun t of extractable ECP in eosinophilic granulocytes is determined by means of extraction with application of a 0.5% Cetyl-trimethyl-ammonium-bromide sol ution. The total amount of extractable ECP in eosinophils amounts to 0.4-17 pg/eosinophil. Results showed a slight tendency to decrease with higher eo sinophil counts. Irrespective of their concentration in blood, eosinophils were demonstrated to release 2-4% of their total ECP amount. It is conclude d from experiments in this study that release during in vitro clotting or m easuring the total amount of extractable ECP does not yield any additional diagnostic value in detecting the degree of activation in eosinophils.