Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae

High-affinity hexose transport is required for efficient utilization of low hexose concentrations by the baker's yeast Saccharomyces cerevisiae. These low concentrations occur during the late exponential phase of batch growth on hexoses, during hexose-limited chemostat or fed-batch culture, or durin g growth on sugars such as sucrose and raffinose that are hydrolysed to hex oses outside the cell. The expression of the Hxt7 high-affinity glucose tra nsporter of S. cerevisiae was examined during batch growth on glucose mediu m in a wild-type strain and a strain expressing only HXT7 (i.e. with null m utations in HXT1-HXT6). In the wild-type strain, HXT7 transcription was rep ressed at high glucose and was detected when the glucose in the culture app roached depletion. In the HXT7-only strain, transcription of HXT7 was const itutive throughout the glucose growth phase and was increased further at lo w glucose concentrations. After glucose depletion, the levels of HXT7 mRNA declined rapidly in both strains. In contrast, the Hxt7 protein was relativ ely stable after glucose depletion. By monitoring the subcellular localizat ion of an Hxt7::GFP fusion protein it was observed that Hxt7 was localized in the plasma membrane, even when expressed at high glucose concentrations in the HXT7-only strain. After glucose depletion Hxt7 was gradually endocyt osed and targeted to the vacuole for degradation. The Hxt7::GFP fusion prot ein was a fully functional hexose transporter with a catalytic centre activ ity of approximately 200/sec. It is concluded that repression of HXT7 and d egradation of Hxt7 at high glucose concentrations is dependent on a high gl ucose transport capacity. Copyright (C) 2001 John Wiley & Sons, Ltd.