Determination of acetone in cow's raw milk by flow injection and gas chromatographic methods

An automatic flow injection (FIA) and two possible reference gas chromatogr aphic (liquid and headspace sampling GC) methods including appropriate samp le preparation were developed and validated for determination of acetone in milk. The methods were tested by preserved raw milk samples. The FIA results were compared with data obtained by GC determinations. It w as found that FIA procedure is suitable for rapid automated measurement of acetone in the range of 0-5 mmol l(-1) in milk, therefore it is a promising analytical method for ketosis monitoring in dairy farms.