Development of a class-specific immunoassay for the type I pyrethroid insecticides

A general enzyme-linked immunosorbent assay was developed for the type I py rethroid insecticides, such as permethrin, phenothrin, resmethrin and biore smethrin. Polyclonal antibodies were generated by immunizing with a permeth rin derivative, 2,2-dimethyl-3-(5 ' -carboxy-pent-1-en-yl)cyclopropanecarbo xylic acid-(3-phenoxybenzyl)ester conjugated with thyroglobulin, bovine ser um albumin or ovalbumin. Antisera were screened against eight different coa ting antigens. The antibody-antigen combination with the lowest background, and highest sensitivity for permethrin was further optimized and tested fo r solvent and detergent tolerance. The I-50's of the optimized immunoassay were 30 mug/l for permethrin and 20 mug/l for phenothrin, respectively. No cross-reactivities were measured to the type H pyrethroids, such as esfenva lerate, cyfluthrin, cypermethrin, deltamethrin, fenvalerate and fluvalinate . This assay can be used in monitoring studies to distinguish between types I and II pyrethroids. (C) 2001 Elsevier Science B.V. All rights reserved.