Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction

Citation
Sr. Mei et al., Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction, ANAL LETTER, 34(12), 2001, pp. 2063-2076
Citations number
25
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL LETTERS
ISSN journal
00032719 → ACNP
Volume
34
Issue
12
Year of publication
2001
Pages
2063 - 2076
Database
ISI
SICI code
0003-2719(2001)34:12<2063:AOU8BC>2.0.ZU;2-G
Abstract
Urinary 8-hydroxydeoxyguanosine (80HdG) has been considered as an excellent marker of individuals at high risk of developing cancer. Until now, urinar y 80HdG has largely been measured by high-performance liquid chromatography with electrochemical detection. A new method for the analysis of urinary 8 0HdG by high-performance capillary electrophoresis has been developed and o ptimized in our laboratory. A single step solid-phase extraction procedure was optimized and used for extracting 80HdG from human urine. Separations w ere performed in an uncoated silica capillary (50 cm x 50 tm i.d.) using a P/ACE MDQ system with UV detection. The separation of 80HdG from interferin g urinary matrix components is optimized with regard to pH, applied voltage , pressure injection time and concentration of SDS in running buffer. The d etection limit of this method is 0.4 mug/ml, the linear range is 0.8-500 mu g/ml, the correlation coefficients levels is better than 0.999. The develop ed method is simple, fast and good reproducibility, furthermore, it require s a very small injection volumes and low costs of analysis, which makes it possible to provide a new noninvasive assay for an indirect measurement of oxidative DNA damage.