Sr. Mei et al., Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction, ANAL LETTER, 34(12), 2001, pp. 2063-2076
Urinary 8-hydroxydeoxyguanosine (80HdG) has been considered as an excellent
marker of individuals at high risk of developing cancer. Until now, urinar
y 80HdG has largely been measured by high-performance liquid chromatography
with electrochemical detection. A new method for the analysis of urinary 8
0HdG by high-performance capillary electrophoresis has been developed and o
ptimized in our laboratory. A single step solid-phase extraction procedure
was optimized and used for extracting 80HdG from human urine. Separations w
ere performed in an uncoated silica capillary (50 cm x 50 tm i.d.) using a
P/ACE MDQ system with UV detection. The separation of 80HdG from interferin
g urinary matrix components is optimized with regard to pH, applied voltage
, pressure injection time and concentration of SDS in running buffer. The d
etection limit of this method is 0.4 mug/ml, the linear range is 0.8-500 mu
g/ml, the correlation coefficients levels is better than 0.999. The develop
ed method is simple, fast and good reproducibility, furthermore, it require
s a very small injection volumes and low costs of analysis, which makes it
possible to provide a new noninvasive assay for an indirect measurement of
oxidative DNA damage.