Quantitative determination of Fluconazole in capsules and IV solutions by UV spectrophotometric methods

Quantitative determination of Fluconazole in IV solutions was carried out b y UV spectrophotometric method using the absorbance values at 261.6 nm and the second order derivative absorbance values at 274 nm. Distilled water wa s used as solvent throughout the study. The concentration range was 4.0 x 1 0(-4)-1.5 x 10(-3) M for the two spectrophotometric methods. While detectio n limit was 4.37 mug/mL in direct UV method. it was 2.63 mug/mL in derivati ve spectrophotometric method. In addition, the slope of the calibration curve in derivative spectrophotom etry was higher than the slope obtained by direct UV method. Percent recove ry values at 261.6 nm were 97.85% for capsules, 96.95% for IV solutions res pectively; but these values were 97.20% for capsules and 104.43% for IV sol utions in second order derivative spectrophotometry at 274 nm (n = 5). RSD values at 274 nm in the second order derivative spectrophotometric method w ere 0.52 for capsules and 0.92 for IV solutions. RSD values showed that bot h spectrophotometric methods were reproducible. In capsules, the two spectr ophotometric methods were performed and found to be comparable to the non-a queous titrimetric method which was applied by using anhydrous acetic acid as solvent and perchloric acid as titrant. The obtained results were compar ed to each other.