Alterations in intracellular Zn2+ concentrations are believed to play a cru
cial role in modulating apoptosis. The observation that Zn2+ deficiency can
induce cell death both in vivo and in vitro has been attributed to the fac
t that exchange of Zn2+ for Ca2+ and Mg2+ within the nuclei may directly ac
tivate endogenous endonucleases therefore inducing DNA fragmentation indepe
ndent of cytoplasmic factors. Here we show that the membrane-permeable zinc
chelator, N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) induces
translocation of cytochrome c from the mitochondrial intramembranous space
into the cytosol in human peripheral blood T lymphocytes (PBL) with subseq
uent activation of caspases-3, -8, and -9. Pretreatment of T lymphocytes wi
th caspase inhibitors Z-VAD.fmk or DEVD.fmk prevented DNA fragmentation in
response to TPEN indicating that apoptosis triggered by zinc deficiency is
entirely dependent on activation of caspase family members. The release of
cytochrome c and activation of downstream caspases precedes changes in the
mitochondrial transmembrane potential (Delta Psim). Therefore, cytoplasmic
and mitochondrial events are critical to this process.