Multipulse feed strategy for glycerol fed-batch fermentation - A steady-state nonlinear optimization approach

During glycerol fed-batch fermentation, the process could be divided into m ultiple equal subintervals, and the feed operation was performed in pulse f orm at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determ ined by a general nonlinear optimization approach to maximize the final gly cerol productivity and still control the residual glucose at a low concentr ation. The experimental results in a 600-mL Airlift Loop Reactor showed tha t the tested data with this strategy agreed well with the corresponding mod el prediction, and that the feed mode with nonlinear optimization could imp rove the glycerol productivity significantly compared with those determined just by limited experimental optimization in previous studies.