Platelet-derived growth factor stimulates the formation of Versican-Hyaluronan aggregates and pericellular matrix expansion in arterial smooth musclecells
Sp. Evanko et al., Platelet-derived growth factor stimulates the formation of Versican-Hyaluronan aggregates and pericellular matrix expansion in arterial smooth musclecells, ARCH BIOCH, 394(1), 2001, pp. 29-38
Hyaluronan and versican-rich pericellular matrices form around arterial smo
oth muscle cells (ASMC) preferentially during the detachment phase of proli
feration and migration. PDGF is a potent mitogen and chemotactic agent for
ASMC and also stimulates the production of extracellular matrix molecules w
hich may regulate the proliferative and migratory capacity of the cells. We
have examined the effect of PDGF on the formation of hyaluronan-dependent
pericellular matrices, and on the synthesis and interaction of several majo
r pericellular coat constituents. As demonstrated using a particle exclusio
n assay, PDGF stimulated the formation of pericellular matrices and was see
n both in an increased proportion of cells with a coat and a greater coat s
ize. This increase was accompanied by a transient increase in hyaluronan sy
nthase 2 (HAS2) expression and an increase in hyaluronan synthesis and poly
mer length. PDGF also increased the synthesis of versican and link protein
as measured at the mRNA and protein levels. The amount of native versican-h
yaluronan aggregates and link-stabilized aggregate was also increased follo
wing PDGF treatment. Time lapse imaging showed that pericellular matrix for
mation occurred around trailing cell processes prior to their detachment. T
hese data suggest that PDGF modulates the synthesis and organization of ASM
C pericellular coat-forming molecules such as versican, hyaluronan, and lin
k protein, which leads to extracellular matrix expansion and alterations in
ASMC phenotype. (C) 2001 Academic Press.