Truncation of WT1 results in downregulation of cyclin G1 and IGFBP-4 expression

Mutations in the WT1 gene are found in a subset of Wilms' tumours and in ce rtain other disorders such as Denys-Drash syndrome. The WT1 gene product is a zinc finger transcription factor for which many target genes have been s uggested. Here we utilise gene targeting to generate cells containing only truncated forms of WT1, in which the DNA-binding region is disrupted. Exami nation of gene expression in these cells using cDNA macroarrays suggests tw o novel WT1 transcriptional targets, cyclin G1 (Ccng1), and insulinlike gro wth factor binding protein 4 (Igfbp4). (C) 2001 Academic Press.