Production of a desulfurization biocatalyst by two-stage fermentation and its application for the treatment of model and diesel oils

For the production of oil-desulfurizing biocatalyst, a two-stage fermentati on strategy was adopted, in which the cell growth stage and desulfurization activity induction stage were separated. Sucrose was found to be the optim al carbon source for the growth of Gordonia nitida CYKS1. Magnesium sulfate was selected to be the sulfur source in the cell growth stage. The optimal ranges of sucrose and magnesium sulfate were 10-50 and 1-2.5 g L-1, respec tively. Such a broad optimal concentration of sucrose made the fed-batch cu lture easy, while the sucrose concentration was maintained between 10-20 g L-1 in the actual operation. As a result, 92.6 g L-1 of cell mass was acqui red by 120 h of fed-batch culture. This cell mass was over three times high er than a previously reported result, though the strain used was different. The desulfurization activity of the harvested cells from the first stage c ulture was induced by batch cultivation with dibenzothiophene as the sole s ulfur source. The optimal induction time was found to be about 4 h. The res ting-cell biocatalyst made from the induced cells was applied for the deep desulfurization of a diesel oil. It was observed that the sulfur content of the diesel oil decreased from 250 mg-sulfur L-oil(-1) to as low as 61 mg-s ulfur L-oil(-1) in 20 h. It implied that the biocatalyst developed in this study had a good potential to be applied to a deep desulfurization process to produce ultralow-sulfur fuel oils.