Effect of a large deletion of the basic domain of mi transcription factor on differentiation of mast cells

The mi transcription factor (MITF) is a basic-helix-loop-helix-leucine zipp er transcription factor that is important for the development of mast cells . Cultured mast cells (CMCs) of mi/mi genotype express abnormal MITF (mi-MI TF), but CMCs of tg/tg genotype do not express any MITFs. It was previously reported that mi/mi CMCs showed more severe abnormalities than tg/tg CMCs, indicating that mi-MITF had inhibitory function. Whereas mi-MITF contains a single amino acid deletion in the basic domain, MITF encoded by mi(ew) al lele (ew-MITE) deletes 16 of 21 amino acids of the basic domain. Here the e ffect of a large deletion of the basic domain was examined. In mi(ew)/mi(ew ) CMCs, the expression pattern of genes whose transcription was affected by MITF was comparable to that of tg/tg CMCs rather than to that of mi/mi CMC s. This suggested that ew-MITF lacked any functions. The part of the basic domain deleted in ew-MITF appeared necessary for either transactivation or inhibition of transactivation. (Blood. 2001;98:2577-2579) (C) 2001 by The A merican Society of Hematology.