CD34(+) cell collection efficiency does not correlate with the pre-leukapheresis hematocrit

One hundred and seventy-seven large-volume leukapheresis procedures perform ed on 91 patients over a 15 month period were reviewed to see if the pre-ap heresis hematocrit (Het) affected the CD34(+) cell collection efficiency (C E) of the Fenwal CS 3000 Plus cell separator. The Hct was 0.174-0.461 (medi an 0.317), and the peripheral blood CD34(+) cell count 2-2487 per mul (medi an 21). The total CD34(+) cell quantity collected was 3.0-2677.2 x 10(6) (m edian 113.0). Based on the number of CD34(+) cells contained in the blood v olume processed (23.3-37303.2 x 10(6); median 318.0), the CE was 1.7-87.5% (median 30.3). No correlation was found between the Hct and CE (r(2) = 0.00 34; P = 0.44) or the total CD34(+) cell quantity collected (r(2) = 0.0040; P = 0.40). CEs for Hct <0.25 (median CE 36%), Het 0.25-0.299 (median CE 30% ) and Hct 0.30 (median CE 30%) were comparable. As expected, highly signifi cant correlations were seen between the CD34+ cell quantities collected and quantities processed (r(2) = 0.59; P < 10(-6)) as well as the peripheral b lood CD34+ cell counts (r(2) = 0.60; P < 10(-6)). We conclude that the mini mum acceptable Hct or hemoglobin level for leukapheresis should be dictated by clinical circumstances because it does not affect stem cell collection.