F. Pi et al., Effects of adenosine on ischaemia-reperfusion injury associated with rat pancreas transplantation, BR J SURG, 88(10), 2001, pp. 1366-1375
Background: During cold preservation, cellular consumption of adenosine tri
phosphate leads to the accumulation of nucleotides and nucleosides. The pre
cise role of adenosine in modulating the inflammatory response of cold-pres
erved pancreas after reperfusion remains to be elucidated. The aim of this
study was to assess the influence of adenosine on the inflammatory response
associated with the process of ischaemia-reperfusion in rat pancreas trans
plantation.
Methods: The effect of adenosine from preservation solution on the levels o
f high-energy nucleotides and their breakdown products after cold ischaemic
preservation was determined. In addition, the inflammatory response associ
ated with the process of ischaemia-reperfusion in pancreas transplantation
was quantified with and without pretreatment with the adenosine antagonist
theophylline, and during preservation of the organ in University of Wiscons
in solution with and without adenosine.
Results: Adenosine from preservation solution is able to modify the nucleot
ide and nucleoside content of preserved pancreas, indicating that adenosine
is incorporated and metabolized in tissue. Administration of the adenosine
antagonist to transplanted rats moderated the increases in nitrite and nit
rate, myeloperoxidase activity and lipoperoxidation levels in the pancreas.
Conclusion: Adenosine in the preservation solution may enhance the inflamma
tory response in rat pancreas transplantation.