Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports

Authors
Nishiguchi, S Yamada, K Fuji, Y Shibatani, S Toda, A Nishimura, SI Nishimura, SI
Citation
S. Nishiguchi et al., Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports, CHEM COMMUN, (19), 2001, pp. 1944-1945
Citations number
15
Categorie Soggetti
Chemistry
Journal title
CHEMICAL COMMUNICATIONS
ISSN journal
13597345 → ACNP
Issue
19
Year of publication
2001
Pages
1944 - 1945
Database
ISI
SICI code
1359-7345(20011007):19<1944:HEOSOW>2.0.ZU;2-H
Abstract
Recombinant beta -1,4-galactosyltransferase (beta1,4-GalT) and alpha -2,6-s ialyltransferase (alpha2,6-SiaT) immobilised covalently with activated Seph arose beads were employed for the practical synthesis of a trisaccharide de rivative, Neu5Ac alpha (2 -->6)Gal beta (1 -->4)GlcNAc beta -O-(CH2)(6)-NH2 , on a water-soluble primer having GlcNAc residues through a alpha -chymotr ypsin-sensitive linker.