Occurrence of polycyclic aromatic hydrocarbons in sewage sludge and their contribution to its toxicity in the ToxAlert (R) 100 bioassay

Citation
S. Perez et al., Occurrence of polycyclic aromatic hydrocarbons in sewage sludge and their contribution to its toxicity in the ToxAlert (R) 100 bioassay, CHEMOSPHERE, 45(6-7), 2001, pp. 705-712
Citations number
27
Categorie Soggetti
Environment/Ecology
Journal title
CHEMOSPHERE
ISSN journal
00456535 → ACNP
Volume
45
Issue
6-7
Year of publication
2001
Pages
705 - 712
Database
ISI
SICI code
0045-6535(200111)45:6-7<705:OOPAHI>2.0.ZU;2-Z
Abstract
The contribution of polycyclic aromatic hydrocarbons (PAH) present in sewag e sludge samples to toxicity was investigated. Chemical analysis using gas chromatography-mass spectrometry (GC-MS) and the ToxAlert (R) 100 bioassay, based on the inhibition to Vibrio fischeri, were applied to sludge extract s after purification by column chromatography. The levels of the 16 US EPA PAH analyzed in the sewage sludge samples varied from 17 to 2030 mug kg(-1) . In all samples examined phenanthrene was the most prominent compound. The toxicity data obtained by ToxAlert (R) 100 can be explained by the levels and composition of the different PAH in sewage sludge samples. The present approach can contribute to evaluate the toxicity of sewage sludge. (C) 2001 Elsevier Science Ltd. All rights reserved.