Elevated interleukin-13 in patients with active lupus nephritis

Citation
Xw. Chen et al., Elevated interleukin-13 in patients with active lupus nephritis, CHIN MED J, 114(10), 2001, pp. 1022-1025
Citations number
8
Categorie Soggetti
General & Internal Medicine
Journal title
CHINESE MEDICAL JOURNAL
ISSN journal
03666999 → ACNP
Volume
114
Issue
10
Year of publication
2001
Pages
1022 - 1025
Database
ISI
SICI code
0366-6999(200110)114:10<1022:EIIPWA>2.0.ZU;2-C
Abstract
Objective To investigate the significance of interleukin-13 ( IL-13) in pat ients with active lupus nephritis (LN). Methods Ten healthy volunteers and 16 patients with active LN were included in this study. The protein level of IL-13 in plasma was examined by enzyme linked immunosorbent assay (ELISA), and gene expression of IL-13 in periph eral blood mononuclear cells ( PBMCs) by reverse transcription polymerase c hain reaction (RT-PCR). Expression of IL-13 mRNA in renal tissue was studie d by in situ hybridization (ISH) techniques. Results The level of IL-13 in plasma and the expression of IL-13 mRNA in PB MCs were significantly higher in LN patients than those in the controls ( P < 0.001). Increased expression of IL-13 mRNA was detected in renal tissue of active LN patients compared to those in the controls ( P < 0.001). Analy sis of the linear correlation indicated that the level of IL-13 mRNA in the tubulointerstitial area in patients with active LN correlated with the con centration of serum creatinine (Scr), the glomerular activity index (GAI), the activity index of tubulointerstitium, and the level of serum C-3 ( P < 0.05 for each). Conclusion The elevation of IL-13 may play an important role in the molecul ar pathogenesis of active LN.