The new monoclonal antibodies against the bovine sperm

Monoclonal antibodies (mAbs) recognising the antigens of spermatozoa could be efficiently used for the study of immunology processes during the sperma togenesis and fertilisation. In this study mAbs showing different reaction patterns on bull and boar sperms have been prepared. BALB/c mice were immun ised with whole bull sperm, and their spleen cells were fused with SP2/0 my eloma cells in the fusion experiment, resulting in the generation of fiftee n mAbs. The antibodies tested in indirect immunofluorescence assay were spe cific for two antigens localised at different parts of sperm surface. Five mAbs are reactive on the whole sperm surface, 3 mAbs recognise antigenic si tes on the proximal part of sperm head, 3 mAbs on acrosome, 2 mAbs on the t ail of sperm (principal piece), 1 mAb on the distal region of the head and 1 mAb was bound to the head and middle part of tail.