K. Lind et al., Comparison of protein adsorption patterns onto differently charged hydrophilic superparamagnetic iron oxide particles obtained in vitro and ex vivo, ELECTROPHOR, 22(16), 2001, pp. 3514-3521
Protein adsorption patterns of superparamagnetic iron oxides (SPIO) were ev
aluated by two-dimensional electrophoresis (2-DE) after in vitro incubation
of the particles in plasma or serum. SPIO particles having positive (MKK 1
211), negative (MKA 1211), or neutral (MKG 1411) charge were used. Protein
adsorption patterns of different charged SPIO particles acquired in vitro a
nd recollected 5 min after intravenous injection into rats (ex vivo) were c
ompared. For the uncharged MKG 1411 particles, the differences of protein a
dsorption patterns were negligible and only minor differences were found fo
r the negatively charged MKA 1211 and positively charged MKK 1211 particles
. A good correlation between in vitro and ex vivo data could be shown. For
the evaluation of protein adsorption patterns of SPIO particles determining
organ distribution and allowing estimation of site-specific delivery (drug
targeting), the currently used protocol for 2-DE analysis could be confirm
ed.