This study attempted to introduce the bio-processes in the conventional sco
uring and bleaching preparation of cotton. The scouring with two types of p
ectinases, acting under acidic and alkaline conditions respectively, was as
efficient as the chemical process in terms of obtained adequate water abso
rbency of the fabrics. The necessity of surfactants application in scouring
was outlined. Bleaching of the fabrics was performed with hydrogen peroxid
e, which was enzymatically produced by glucose oxidase during oxidation of
glucose. The aeration plays an important role in the enhancement of the enz
yme reaction, so that the quantity of generated peroxide is sufficient to o
vercome the stabilizing effect of the glucose and protein in the subsequent
bleaching. A closed-loop process reusing starch containing desizing baths
in a single step scouring/bleaching operation with enzyme-generated peroxid
e was performed. (C) 2001 Elsevier Science Inc. All rights reserved.