Temporary warm ischaemia, 5/6 nephrectomy and single uranyl nitrate administration - comparison of three models intended to cause renal fibrosis in rats
D. Appenroth et al., Temporary warm ischaemia, 5/6 nephrectomy and single uranyl nitrate administration - comparison of three models intended to cause renal fibrosis in rats, EXP TOX PAT, 53(4), 2001, pp. 316-324
In patients the progression of pathologic renal processes after the treatme
nt of primary disease is a problem of increasing importance and therapeutic
strategies are insufficient till now. The aim of this paper was to search
for rat models of interstitial fibrosis as a basis for testing therapeutic
strategies to prevent end-stage renal failure.
Experiments were done on adult female Wistar rats (Han:Wist) to investigate
long-term consequences of temporary warm ischaemia, 5/6 nephrectomy (5/6 N
X) and sing e uranyl nitrate (UN) administration (0.3 or 0.5 mg/100 g body
wt, intraperitoneally). Observation time was 20 weeks after injury in each
group. Creatinine clearance, urinary protein excretion and hydroxy-proline
(OH-proline) concentration in renal tissue were measured and light microsco
pic investigations were done to characterise both quality and time course o
f long-term renal damage in relation to matched control animals. Temporary
warm ischaemia and 5/6 NX did not cause any fibrotic changes during the 20
weeks observation period.
In conclusion, administration of one single dose of UN is a simple procedur
e to induce interstitial renal fibrosis as an experimental model to investi
gate therapeutic strategies for their prevention.