Egg shell ultrastructure of the fish nematode Huffmanela huffmani (Trichosomoididae)

The egg shell of Huffmanela huffmani Moravec, 1987 forms three main layers: an outer vitelline layer, a middle chitinous layer, and an inner lipid lay er. The vitelline layer, forming the superficial projections of the egg she ll, comprises two parts: an outer electron-dense, and an inner electron-luc id part. The chitinous layer is differentiated into three parts: an outer h omogenous electron-dense part, a lamellated part, and an inner electron-den se net-like part. The lipid layer comprises an outer net-like electron-luci d part, and an inner homogenous electron-lucid part. The polar plugs are fo rmed by electron-lucid material with fine electron-dense fibrils.