A. Yamamoto et al., Molecular cloning and expression of the cDNA encoding feline granulocyte colony-stimulating factor, GENE, 274(1-2), 2001, pp. 263-269
Both genomic DNA and cDNA of the feline granulocyte colony-stimulating fact
or (G-CSF) gene were cloned from CRFK cells. Southern blot analysis showed
that the haploid genome contains a single copy of the G-CSF gene. The RT-PC
R analysis of several feline cell lines revealed expression of G-CSF mRNA i
n response to lipopolysaccharide stimulation. Sequence analysis of genomic
and cDNA clones indicated that the intron-exon junction structure is conser
ved between the human and the feline G-CSF genes. The G-CSF coding region e
ncodes a predicted protein of 195 amino acids including a signal sequence o
f 21 amino acids. The feline G-CSF amino acid sequence shares a high degree
of identity with the canine (90.8%), human (87.4%), ovine (83.9%), bovine
(82.8%), porcine (80.5%), murine (70.7%) and rat (66.8%) G-CSF. The feline
G-CSF expressed in insect cells using recombinant baculovirus vector was bi
ologically active as measured in a proliferation assay using NFS-60 cells a
nd an induction assay of leukocytes in cats. (C) 2001 Elsevier Science B.V.
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