Establishment of Polycomb silencing requires a transient interaction between PC and ESC

Two distinct types of Polycomb complexes have been identified in flies and in vertebrates, one containing ESC and one containing PC. Using LexA fusion s, we show that PC and ESC can establish silencing of a reporter gene but t hat each requires the presence of the other. In early embryonic extracts, w e find PC transiently associated with ESC in a complex that includes EZ, PH O, PH, GAGA, and RPD3 but not PSC. In older embryos, PC is found in a compl ex including PH, PSC, GAGA, and RPD3, whereas ESC is in a separate complex including EZ, PHO, and RPD3.