STABILITY STUDY OF CIPROFLOXACIN HYDROCHLORIDE USING REVERSED-PHASE HPLC

In this study, the stability properties of ciprofloxacin hydrochloride were investigated with the aim at formulating hard capsules. A HPLC m ethod is presented and validated for the determination of this molecul e and its degradation products. This procedure is a modified method of the European Pharmacopoeia. The analysis has been performed on a Lich rospher RP-18 column at 40 degrees C with UV detection at 278 nm. The mobile phase was methanol/phosphoric acid 0.245% in water first adjust ed to pH 3.0 with triethylamine (12:88, v/v). The results have shown t hat the molecule studied is proof against heat and humidity but is sli ghtly sensitive to photodegradation. The main degradation product iden tified is the desethyleneciprofloxacin.