Preparative isolation and purification of salidroside from the Chinese medicinal plant Rhodiola sachalinensis by high-speed counter-current chromatography

High-speed counter-current chromatography was applied to the isolation and purification of salidroside from the Chinese medicinal plant Rhodiola sacha linensis A. Bor. The crude salidroside was obtained by extraction with meth anol from Rhodiola sachalinensis A. Bor. Preparative high-speed counter-cur rent chromatography with a two-phase solvent system composed of n-butanol-e thyl acetate-water (2:3:5, v/v) was successfully performed yielding salidro side (32 mg) at 98% purity from 250 mg of the crude extract in a one-step s eparation. (C) 2001 Elsevier Science B.V. All rights reserved.