Consideration on peak shape in a batch-type chemiluminescence detection cell for capillary electrophoresis

Peak areas, peak heights, and apparent theoretical plate numbers were exami ned as a function of sample injection times by use of the batch-type CL det ection cell. Comparing the experimental data with those obtained by absorpt ion detector, some considerations were carried out about the peak shape. Th e peak shape in CL detection was influenced by not only concentration distr ibution of sample in a sample zone but also sample diffusion and CL reactio n at the capillary outlet. The sample injection time of ca. 35 s was recomm ended for the present CE with CL detector. The injection time much influenc ed peak shape as well as sensitivity in the CL detection cell. (C) 2001 Els evier Science B.V. All rights reserved.