Determination of bioactive diterpenoids from Andrographics paniculata by micellar electrokinetic chromatography

The present paper describes the development of a micellar electrokinetic ch romatographic (MEKC) method for simultaneous determination of andrographoli de, deoxyandrographolide and neoandrographolide in ethanol extracts of Andr ographis paniculata. Separations were carried out in a fused-silica capilla ry tube with UV detection at 214 nm. Good separation was achieved using a 2 0 mM borate buffer, containing 20 mM sodium dodecyl sulphate and 10 mM sodi um cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperatur e of 35 degreesC and a hydrodynamic injection of 5 s. The method was valida ted with good correlation coefficients obtained (0.9986-0.9989) while relat ive standard deviation (RSD) of migration time was between 1.14 and 2.42. I t is concluded that this method could be used for speedy and accurate quali tative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products. (C) 2001 Elsevier Science B.V. All rights re served.