Protective role of Delphinium denudatum (Jadwar) against morphine induced tolerance and dependence in mice

Chronic treatment with Delphinium denudatum (Dd) (Jadwar) (family: Ranuncul aceae, 200-1600 mg/kg) suppressed morphine withdrawal jumps in a dose-depen dent manner, a sign of the development of dependence to opiate as assessed by naloxone (2 mg/kg) precipitation withdrawal on day 10 of testing in mice . Repeated administration of Dd (200-1600 mg/kg) for 9 days attenuated the development of tolerance to the analgesic effect of morphine (10 mg/kg), al so produces significant change in tail-flick latency from the saline pretre ated group in a dose-dependent manner. (C) 2001 Published by Elsevier Scien ce Ireland Ltd.