Anatomically compartmentalized human immunodeficiency virus replication inHLA-DR+ cells and CD14(+) macrophages at the site of pleural tuberculosis coinfection

Citation
Sd. Lawn et al., Anatomically compartmentalized human immunodeficiency virus replication inHLA-DR+ cells and CD14(+) macrophages at the site of pleural tuberculosis coinfection, J INFEC DIS, 184(9), 2001, pp. 1127-1133
Citations number
57
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF INFECTIOUS DISEASES
ISSN journal
00221899 → ACNP
Volume
184
Issue
9
Year of publication
2001
Pages
1127 - 1133
Database
ISI
SICI code
0022-1899(20011101)184:9<1127:ACHIVR>2.0.ZU;2-V
Abstract
This study examined the impact of the host inflammatory microenvironment as sociated with localized tuberculosis (TB) on human immunodeficiency virus t ype 1 (HIV-1) replication within lymphocytes and macrophages in vivo. Paire d plasma and pleural fluid samples from HIV-1-infected individuals with ple ural TB (n = 9) were analyzed. Detection of host proteins incorporated into the HIV-1 envelope by immunomagnetic capture analysis provided insight int o the phenotype of cells supporting HIV-1 replication. The results indicate d that the 4.0-fold greater median HIV-1 load in pleural fluid, compared wi th median load in plasma (P < .01), was derived in part from viral replicat ion within HLA-DR+ cells, CD26(+) lymphocytes, and, importantly, CD14(+) ma crophages. Greatly increased local concentrations of proinflammatory cytoki nes and immune activation markers in the pleural space correlated with the virologic findings. In summary, HIV-1 replication was increased at sites of Mycobacterium tuberculosis coinfection within activated cells, including l ymphocytes and CD14(+) macrophages.