Involvement of p38-mitogen-activated protein kinase in staphylococcus aureus-induced neutrophil apoptosis

Apoptosis occurred in human neutrophils within an hour of exposure to viabl e serum-opsonized Staphylococcus aureus, as indicated by appearance of cell s with condensed nuclei, fragmented DNA, and increased phosphatidylserine e xposure. In contrast, serum-opsonized, heat-killed S. aureus did not induce apoptosis. This discrepancy could not be explained by differences in bacte rial uptake or total NADPH-oxidase activity. Suppressing phagocytosis by pr etreating the neutrophils with cytochalasin b or by using nonopsonized bact eria did not prevent apoptosis. A supernatant from bacteria grown for 2 h i n nutrient broth had a strong proapoptotic influence that was abrogated by heat treatment. Exposure to viable S. aureus or supernatant also led to act ivation of p38-mitogen-activated protein kinase in the neutrophils. Inhibit ion of this kinase with SB203580 reduced the apoptosis-inducing capacity of both bacteria and supernatant. We conclude that S. aureus activates p38-mi togen-activated protein kinase,in neutrophils and induces apoptosis, probab ly mediated by a bacteria-derived soluble factor(s).