PRIMARY STRUCTURE OF TROPONIN-I ISOFORMS FROM THE ASCIDIAN HALOCYNTHIA-RORETZI

The solitary ascidian Halocynthia roretzi possesses three types of mus cle: the larval tail striated muscle, the adult heart striated muscle, and the adult body wall smooth muscle, The troponin complex is observ ed in ail types of muscle, and the isoform sequences and expression pa tterns of two of the three troponin components, troponins C and T, hav e been reported, In this study, we have determined cDNA sequences of t he three TnI isoforms from H. roretzi. One of the three isoforms (adul t TnI), expressed in adult body wall smooth muscle and heart muscle, w as composed of 173 amino acids, being similar to vertebrate fast and s low skeletal TnIs in length, The other two isoforms (larval TnI alpha and TnI beta) were isolated from a cDNA library of larvae. Both larval TnIs were composed of 142 amino acids, with truncation amounting to c a, 30 amino acid residues at the C-termini, These larval TnIs are the smallest known TnIs. The position of the last intron of these TnIs was also determined. When compared with vertebrate TnI genes, the last in tron of the ascidian adult TnI gene is located at 6 nucleotides downst ream, and the introns of the two larval TnIs are positioned at 9 nucle otides upstream, These results suggest that H. roretzi TnI is encoded by at least three genes.