Resource competition determines selection of B cell repertoires

Previous experiments with mouse chimeras demonstrated that cellular competi tion for antigen-specific survival signals plays a crucial role in the main tenance of the naive B cell repertoire. Transgenic (Tg) B cell populations in these chimeras have a shortened lifespan and poor competitive abilities as compared to more diverse non-Tg populations in the same mice. We develop a mathematical model to investigate the mechanism of B cell competition. T he model allows for various B cell clones, generated in the bone marrow, to go into the peripheral circulation, where they compete specifically for va rious ligands providing survival signals. In the model we also find the obs erved poor competitive abilities of the Tg repertoire. Investigating the na ture of the competition in the model, we find that most of the competition is "intraspecific" occurring largely within the clone of truly Tg B cells, and within the repertoire of leaky Tg and non-Tg B cells. This is confirmed by analysing a simplified version of the model, which only allows for intr aspecific competition, and resembles a simple ecological model with density -dependent death. The fact that our model accounts for the data, casts doub t on a previous interpretation of the same data arguing that more diverse r epertoires outcompete repertoires of lower diversity. Here, we conclude tha t most of the data can be explained with intraspecific competition, and for mulate an experimental prediction that allows one to distinguish between th e previous interpretation of inter-specific competition between repertoires , and the current interpretation of intraspecific competition. (C) 2001 Aca demic Press.