A human immunodeficiency virus Env inducible transcription system to examine consequences of gp120 expression

Citation
V. Joliot et al., A human immunodeficiency virus Env inducible transcription system to examine consequences of gp120 expression, J VIROL MET, 98(2), 2001, pp. 145-151
Citations number
27
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGICAL METHODS
ISSN journal
01660934 → ACNP
Volume
98
Issue
2
Year of publication
2001
Pages
145 - 151
Database
ISI
SICI code
0166-0934(200111)98:2<145:AHIVEI>2.0.ZU;2-8
Abstract
According to several studies, the HIV-1 envelope gp120 protein and the co-r eceptor CXCR4 play an essential role in HIV-1 induced cell toxicity. Charac terisation of the CD4-independent m7NDK isolate provided the opportunity of studying the effects of direct interactions between m7NDK gp120 and CXCR4. Therefore, an inducible expression system was designed enabling synthesis of HIV-1 Env proteins upon doxycycline induction. Analysis of the expressio n of the env gene of the m7NDK HIV-1 isolate revealed, unexpectedly, that e ven long-term expression of m7NDK gp120 did not result in cytotoxycity in C XCR4-positive or -negative cell lines. This is the first report of a CD4-in dependent HIV-1-protein inducible expression regulated through the Tet-On s ystem and by an alternative splicing. Env inducible expression cell lines c ould constitute a useful cellular tool to undertake analysis of HIV Env pro tein expression. (C) 2001 Elsevier Science B.V. All rights reserved.