Immunohistochemical expression of cell cycle proteins E2F-1, Cdk-2, cyclinE, p27(kip1), and Ki-67 in normal placenta and gestational trophoblastic disease
M. Olvera et al., Immunohistochemical expression of cell cycle proteins E2F-1, Cdk-2, cyclinE, p27(kip1), and Ki-67 in normal placenta and gestational trophoblastic disease, MOD PATHOL, 14(10), 2001, pp. 1036-1042
Citations number
41
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
The role of cell cycle protein expression in gestational trophoblastic dise
ase is poorly understood. In this study we investigated the Immunostaining
patterns of G(1) restriction point and G(1)-S regulatory proteins E2F-1, Cd
k2, cyclin E, p27(kip1), and the proliferation marker Ki-67 on routinely pr
ocessed sections of 29 hydatidiform moles (10 partial moles and 19 complete
moles, Including 9 persistent moles), 7 choriocarcinomas, and 7 normal pla
centas. Ki-67 trophoblast staining decreased with increasing gestational ag
e of the placenta, and showed maximal expression in gestational trophoblast
ic disease. Cyclin-dependent kinase activity, as reflected by Cdk2 expressi
on patterns, also decreased with placental maturation. E2F-1 was uniquely e
xpressed by trophoblasts of moles and choriocarcinoma. Cyclin E was maximal
ly expressed by complete moles and choriocarcinomas, and showed an inverse
relationship with the cyclin-dependent kinase Inhibitor P27(kip1). Abnormal
trophoblastic proliferations may be mediated through interactions of Cdk-2
, E2F-1, cyclin E, and p27(kip1). Overexpression of cyclin E was associated
with more aggressive forms of gestational trophoblastic disease. However,
we did not find distinguishing features between complete moles that spontan
eously resolved after evacuation and persistent moles that required chemoth
erapy. The different expression patterns of cyclin E and E2F-1 in partial a
nd complete moles may be useful in distinguishing these two entities. Furth
ermore, loss of P27(kip1) in malignant trophoblast may represent a necessar
y step in the development of choriocarcinoma.